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Reverse Phase HPLC Columns





MSepax GP-C18
GP-C18 uses full coverage bonded silica packing, which provides exceptionally high stability. The unique mono-functional bonding chemistry of GP-C18 avoids the formation of multiple C18 layers. Such uniform stationary phase allows the separation to achieve high selectivity and high efficiency.

 

Sepax HP-C18
An in line process refractometer allows you to continuously monitor and control the concentration of dissolved solids or water-soluble liquids, saving you both time and money.




Sepax HP-C18(2)
HP-C18(2) uses a proprietary ODS bonding chemistry to a high surface area silica gel to achieve long retention time and high loading capability. The ODS density is specially designed to achieve optimal selectivity for both hydrophobic and hydrophilic compounds.

 


Sepax Bio-C18
The uniform stationary phase allows the separation to achieve high selectivity and high efficiency. Bio-C18 packings of 200 and 300 Å pore size selection are ideal for high resolution mapping of peptides and separation of natural and synthetic peptides and small proteins.









Sepax BR-C18
C18 phase formed by special bonding chemistry for applications in wide range of pH (1.5-10.5).

 






Sepax GP-C8
GP-C8 phase is synthesized with monomeric and fully endcapped chemistry. The uniform octyl stationary phase allows high efficiency with lower hydrophobicity compared to ODS phase. GP-C8 phase is suitable for separating compounds with a wide range of hydrophobicity. It is highly recommended for separating the compounds which are too strongly retained on C18 phases.


ASepax Bio-C8
Bio-C8 phase is made of monomeric and fully endcapped chemistry. The uniform stationary phase allows the separation to achieve high selectivity and high efficiency. Bio-C8 packings of 200 and 300 Å pore size selection are ideal for high resolution mapping of peptides and separation of natural and synthetic peptides and small proteins.

 



Sepax GP-C4

Monomeric and fully endcapped GP-C4 packing is bonded with butyl group that leads to moderate hydrophobicity. GP-C4 columns have the great selectivity and peak symmetry with moderate retention for separations of acidic, neutral and basic organic compounds, such as pharmaceuticals, peptides, and organic acids.






Sepax Bio-C4
Monomeric and fully endcapped GP-C4 packing is bonded with butyl group that leads to moderate hydrophobicity. Bio-C4 packings of 200 and 300 Å pore size selection are ideal for high resolution mapping of peptides and separation of natural and synthetic peptides and small proteins.

 

70
6Sepax GP-Phenyl
GP-Phenyl packing materials are bonded with propyl phenyl groups that enable special interaction with ring structured compounds. The monomeric bonding chemistry gives very high efficiency and high resolution separations. GP-Phenyl phase is suitable for separations of acidic, neutral and basic organic compounds, as well as the pharmaceuticals.








Normal Phase HPLC Columns







Sepax HP-Cyano
Synthesized with monomeric and fully endcapped chemistry, HP-Cyano phase is bonded with propylcyano functional group that allows special interaction with polar compounds. The monomeric bonding chemistry enables high efficiency and high resolution separation of peptide, proteins, acidic, neutral and basic organic compound, and pharmaceuticals.

 



Sepax HP-Amino

Synthesized with polymeric chemistry, HP-Amino phase is bonded with aminopropyl functional group. HP-Amino phase is compatible with versatile mobile phases from non-aqueous solvents, such as hexane/ethyl acetate and chloroform/methanol, to aqueous solutions. It is recommended for separations of sugars, nucleotides, basic organic compounds, as well as the pharmaceuticals.


Sepax HP-Diol
Synthesized with polymeric chemistry, HP-Diol phase is bonded with 1,2-dihydroxypropyl propyl ether functional group that allows special interaction with polar compounds. The polymeric bonding chemistry enables high efficiency and high stability separation of polar pharmaceuticals, peptide, and proteins. HP-Diol phase can also be used as size exclusion separation of biological molecules.

 


Sepax HP-Silica
HP-Silica phase is made of activated hydroxyl (-OH) functional group with the pore size selection of 60, 120, 200, 300, 500, 1000 and 2000 Å and particle size selection of 1.8, 2.2, 3, 5, and 10 µm. Carbon loading is 0.0%. HP-Silica is used as the normal phase as well as HILIC phase. HP-Silica phases are suitable for separations of polar and basic organic compounds, such as vitamins, steroids, as well as pharmaceuticals.

Mixed-mode Phase











Sepax HP-SCX HPLC Columns
HP-SCX is a silica based mixed-mode strong cation exchange phase. The phase has a mixed chemical structure of sulfonic acid and phenyl group. The mixed mode of strong cation-exchange and hydrophobicity offers high selectivity and high resolution separation for cationic/basic and nitrogen containing compounds as well as desirable retention for a variety of weak cationic and neutral organic compounds. A uniform, polymeric bonding of HP-SCX enhances its stability under various separation conditions.
HP-SCX has expanded its applications from the traditional SCX separation of cationic and nitrogen containing compounds to weak cationic and neutral organic molecules. Examples include separation of analytes of amines and polyamines, such as alkaloids, peptides, codeine, cough and cold ingredients

HILIC Phase HPLC Columns

 

 

 

 


P
Sepax HILIC Phases

To solve the challenges of more and more highly polar pharmaceuticals and small biological molecules, Sepax developed a series of chemistries of weak acidic, neutral, and basic stationary phases for separating basic, neutral and acidic compounds of high polarity. Utilizing highest purity and enhanced mechanical stability silica and pure bonding reagents, Sepax HILIC bonded phases have been innovatively and specially designed to ensure maximum surface coverage, resulting in high stability of the stationary omega replika saat phases. The chemistry of monolayer formation is completely controlled that results in very reliable lot-to-lot and column-to-column reproducibility. The uniform, spherical Sepax HILIC particles have a nominal surface area of 300 m²/g with a controlled pore size of 120 Å. Sepax HILIC columns are packed with a proprietary slurry technique to achieve uniform and stable packing bed density for maximum column efficiency.

Polymer Based Phase

 











Sepax PolyRP Columns
PolyRP phases are made of 80% cross-linking PS/DVB spherical particles. Those highly rigid particles have both non-porous and porous structures with the particle size selection of 1, 1.7, 3, 5, and 10 µm. The phase breitling replika structure is phenyl functional group that enables hydrophobic interaction. Compared with silica based reversed phases, PolyRP phases have advantages over applications at extreme pH (1-14) with special selectivity and slightly lower separation efficiency.

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